Thursday, February 26, 2009

Circular oblique illumination (COL) a...






Circular oblique illumination (COL) and the examination of blood www.microscopy-uk.org.uk/mag/artmay04/pjblood.html
Off axis COL illumination www.microscopy-uk.org.uk/mag/artdec04/pjoblique.html
Phase
Contrast and COL combinedIt occurred to me that since the imagery
raised through the employment of full aperture annuli in COL has
subdued tonality, but very high resolution, www.microscopy-uk.org.uk/mag/artnov07/pj-condenser2.html
Circular
Oblique Lighting (Part 1)Technically speaking it is 360 degree Oblique
Illumination OR Circular Oblique Lighting For brevity's sake I shall
refer to it as COL illumination www.microscopy-uk.org.uk/mag/artdec02/pjcol.html
Off
Axis COL UpdateThose who have taken to COL lighting techniques will no
doubt have developed it to their own satisfaction by marrying substage
annuli to suitable objectives www.microscopy-uk.org.uk/mag/artmay08/pj-colupdate.html
Circular
oblique lighting (COL) Part IV: updateI have been constantly impressed
with COL, and especially with the humble achromat: the performance of
which seems to be particularly elevated in comparison www.microscopy-uk.org.uk/mag/artoct03/pjcol4.html
Circular Oblique Lighting Part 2It must be emphasized that there is no definitive COL set-up, www.microscopy-uk.org.uk/mag/artjan03/pjcol2.html
Circular Oblique Lighting (In concluding this final article on COL I'd like to describe a few moments www.microscopy-uk.org.uk/mag/artfeb03/pjcol3.html
A Condenser For All SeasonsSince Phase contrast, Darkfield, and COL are generated by annuli of differing NA www.microscopy-uk.org.uk/mag/artoct07/pj-condenser.html
Topical Tip: Notes on using the LOMO darkfield condenser for both www.microscopy-uk.org.uk/mag/artsep07/dw-lomo-df.html
The Heine condenser www.microscopy-uk.org.uk/mag/artsep03/pjheine.html
Illumination in Microscopy: Why I like darkfield illumination www.microscopy-uk.org.uk/mag/artmar04/godarkfield.html
Notes on adapting a Nikon Labophot 1 stand for LED use www.microscopy-uk.org.uk/mag/artaug07/dw-labophot.html
Homage
to the onion skinPaul James, an artist and mastercraftsman of
microscopy, innovator, and enthusiastic advocate of the COL diaphragm
contributes these four very beautiful www.microscopy-uk.org.uk/mag/artnov03/wdonion.html
Microscopes,
filters, diaphragms and stopsHis diatom pictures with COL diaphragms
are exceptional (the stops he proposes is www.microscopy-uk.org.uk/mag/artdec03/wdonion2.html
Illumination in Microscopy www.microscopy-uk.org.uk/mag/artmar06/go-phase.html
Preservation
of Contrast in the Compound Microscope (Part 2)After thinking about
this for a while I decided to leave the Kohler setup exactly as it was
for best imagery in COL for both photo images and to reduce the www.microscopy-uk.org.uk/mag/artmay07/pj-contrast.html
"Oblique Darkfield" IlluminationEventually, a series of articles in Micscape Magazine about COL (Circular www.microscopy-uk.org.uk/mag/artmay05/twoblique.html
A Case for Thin Coverslips www.microscopy-uk.org.uk/mag/artapr05/pjcoverslip.html
TECHNICAL
TIPS ON THE USE OF THE PRECISE DOUBLE RAZOR BLADES SLICERI take
pictures with the 10 x and 40 x objectives using the COL-D3 contrast
disc that gives a www.microscopy-uk.org.uk/mag/artapr04/wdslicerb.html
Dicotyledon stem2 - With the COL-D1 filter some tissues are differentiated and the vascular bundle www.microscopy-uk.org.uk/mag/artfeb04/wdstem.html
So
a scratch ruins a microscope objective?It is important to note that the
comparisons vary to a lesser degree in COL and oblique simply because
the outer regions of the front optic are being www.microscopy-uk.org.uk/mag/artjun04/pjso.html
The Heine condenser (part 2) www.microscopy-uk.org.uk/mag/artnov03/pjheine2.html
Microscopy-UK Micscape Microscopy and Microscopes MagazineDF can offer higher contrast than COL for many applications www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artmar04/godarkfield.html
Microscopic fauna - some lifestyles www.microscopy-uk.org.uk/mag/artsep05/wdparasite4.html
Imaging problems with certain Digital cameras www.microscopy-uk.org.uk/mag/artdec03/pjimaging.html
Nature Center Microscope with Darkfield CapabilityThis situation could be used to give circular oblique lighting (COL) www.microscopy-uk.org.uk/mag/artmar03/tcnaturescope.html
Topical tip: Practical notes on using off-axis oblique www.microscopy-uk.org.uk/mag/artdec08/dw-obliquetip.html
No formalin, no mercury fixatives www.microscopy-uk.org.uk/mag/artsep03/wdfixers2.html
Alternative mounting media revisited www.microscopy-uk.org.uk/mag/artjun04/wdmtmedia.html
Micscape Library Index page 3Aug www.microscopy-uk.org.uk/mag/libindex3.html
A tour round a Leitz Diaplan microscope www.microscopy-uk.org.uk/mag/artjan09/dw-diaplan.html
Preservation
of Contrast in the Compound Microscope (Part 1)There are many articles
written about the contrast enhancing techniques of Phase Contrast, DIC,
DF, COL, Oblique etc but very few are concerned with the www.microscopy-uk.org.uk/mag/artapr07/pj-contrast.html
Fitting
an AO (American Optical) Substage with a Leitz Heine CondenserBut being
anxious to explore COL, and not having dry, high power Dark Field www.microscopy-uk.org.uk/mag/artaug05/snfitao.html
ARCHIVE
ISSUE: Micscape Magazine, exploring the miniature worldSubstage polar
control lighting units (Part 2) - Paul James (UK) describes his '
efforts to generate a hybrid illumination technique between COL and
Phase www.microscopy-uk.org.uk/mag/nov07ind.html
ARCHIVE
ISSUE Micscape Magazine, exploring the miniature worldOff axis COL
illumination - Paul James (UK) describes how to achieve this '
distinctive' form of illumination illustrated with images of soil
amoebae www.microscopy-uk.org.uk/mag/dec04ind.html
Miscellaneous GalleryContrast enhanced COL image at around x 1200 of a lively critter resting www.microscopy-uk.org.uk/mag/artjan07/pj-miscella.html
Safe microscopic techniques for amateurs www.microscopy-uk.org.uk/mag/artaug03/wdpart4.html
StentorsDepth: collected near the shore, at less than 6 inches depth www.microscopy-uk.org.uk/mag/artjul07/hw-stentor.html
Random Collecting (of a freshwater plankton sample from Lake Superior)Random Collecting www.microscopy-uk.org.uk/mag/artnov05/hwrandom.html
Optimal
output from the AbbeThis image above is a curious mixture of BF, COL
and DF generated from the concentrically zoned lighting, which may not
be considered to be a viable or www.microscopy-uk.org.uk/mag/artapr08/pj-optimal.html
A Monastery PondDepth: collected near the shore, at less than a meter www.microscopy-uk.org.uk/mag/artdec05/hwmonastery.html
Voracious
Soil AmoebaeThese images were captured using various illumination
techniques such as brightfield, phase contrast and COL, implemented at
various times using the Zeiss www.microscopy-uk.org.uk/mag/artfeb05/pjvoraceou.html
THE LEITZ DIALUX 1Jan 25, 2006 www.microscopy-uk.org.uk/mag/artjan06/pjleitz.html
Reducing
lighting anomalies in low power darkfield illuminationAn annulus of the
type used in Phase Contrast or COL illumination is ideal, more
especially if its NA is just beyond that of the objective in use www.microscopy-uk.org.uk/mag/artoct06/pj-reducing.html
The Mathias arrow, a versatile oblique lighting technique www.microscopy-uk.org.uk/mag/artoct03/wdoblique.html
Colour Phase Contrast MicroscopyTo gain some contrast, the section is illuminated with annular illumination (COL ) NA0 www.microscopy-uk.org.uk/mag/artmar04/rvwphase.html
Used
Microscope Phase CondensersThe Brightfield apertures can be seen ( one
of 3) and thus some homemade COL annuli can be installed as well,
making for a very versatile condenser www.microscopy-uk.org.uk/mag/artjul07/pj-phasecond.html
Water objectives www.microscopy-uk.org.uk/mag/artoct04/pjwater.html
Aptenia cordifolia, a short illustrated monograph www.microscopy-uk.org.uk/mag/artaug04/wdaptenia.html
Some stains for amateur microscopyPhilodina alive, taken through a COL-D3 filter www.microscopy-uk.org.uk/mag/artmay04/wdstains.html
ARCHIVE
ISSUE: Micscape Magazine, exploring the miniature worldOff-axis COL
update - Paul James (UK) shares observations on his further studies of
a variant of COL using a 40x objective in particular www.microscopy-uk.org.uk/mag/may08ind.html
The
Eye and Illumination with the Brightfield MicroscopeAbove COL
illumination technique using an example to demonstrate that a small www.microscopy-uk.org.uk/mag/artdec05/pjeye.html
A
tour around the Zeiss Photomicroscope IIImulti-technique transmitted
light studies without condenser changeout, the phase condenser allows
phase, some darkfield (or DIC if you can afford it), COL, www.microscopy-uk.org.uk/mag/artnov07/dw-pm3.html
Hover Flies - All About HoverfliesStubbs, A www.microscopy-uk.org.uk/mag/artmay07/cd-hoverflies.html
Microscopic fauna - some lifestyles www.microscopy-uk.org.uk/mag/artoct05/wdparasite5.html
ARCHIVE
ISSUE Micscape Magazine, exploring the miniature worldCOL's
renditioning of blood - Paul James (UK) shares some striking images of
blood using circular oblique illumination with notes on how they were
created www.microscopy-uk.org.uk/mag/may04ind.html
Eosin as a nuclear stain in botany and as an economical substitute www.microscopy-uk.org.uk/mag/artapr06/wd-eosin.html
Microscopy UK / Micscape - Microscopy Articles LibraryApr www.microscopy-uk.org.uk/mag/libindex.html
Microscopy-UK Micscape Microscopy and Microscopes MagazineMicroscopy-UK www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artaug07/dw-labophot.html
untitledThis book is a great one with lots of information and pictures www.microscopy-uk.org.uk/topten_algae.html
MODELO
MICSCAPE PROPIOA number of contrast devices (Rheinberg discs, Black
field discs, COL discs, and the Mathias arrow), could had been used to
impart color, or relief to some www.microscopy-uk.org.uk/mag/imgapr06/Eosin-english.html

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